Media - LB (Luria-Bertani)

by A. Untergasser (contact address and download at www.untergasser.de/lab)
Version: 1.0 - Print Version (.PDF)

This is a very basic recipe used in any lab. It is probably THE most used bacteria medium.
Costs: 0.85 euro/liter

Composition:

1.0 %	Tryptone
0.5 %	Yeast Extract
1.0 %	NaCl

10 g	Tryptone
5 g	Yeast Extract
10 g	NaCl

add 950 ml water

adjust the pH to 7.0 with 5 M NaOH

add water to 1 liter

Autoclave for 20 min at 120 °C

Let cool to ca. 55 °C before adding antibiotics

Store at room temperature or 4 °C

Plates:

Prepare LB as described above, but add before autoclaving:

1,5 % Agar

15 g Agar

Materials needed:

Tryptone (# T1332.1000) by Duchefa
Yeast Extract (# Y1333.1000) by Duchefa

Known Issues:

I always use LB with half of the NaCl concentration and I have no clue why.
I never ajusted the pH because I am lazy and the bacteria didn't mind it.

References and Comments:

This is a known recipe as it can be found for example in "Molecular Cloning: A Laboratory Manual".

How to cite this page in publications:

This document can be cited like this:

Untergasser A. “Media - LB (Luria-Bertani)” Untergasser's Lab. Summer 2006 (include here the date when you accessed these page).
<http://www.untergasser.de/lab/media/media_lb_v1_0.htm>.

Please Do Not Reprint This Article:

This article is copyrighted. Please do not reproduce this article in whole or part, in any form, without obtaining my written permission.