Media - LB (Luria-Bertani)

by A. Untergasser (contact address and download at
Version: 1.0 - Print Version (.PDF)

This is a very basic recipe used in any lab. It is probably THE most used bacteria medium.
Costs: 0.85 euro/liter


1.0 % Tryptone
0.5 % Yeast Extract
1.0 % NaCl
10 g Tryptone
5 g Yeast Extract
10 g NaCl
  -- add 950 ml water
adjust the pH to 7.0 with 5 M NaOH
  -- add water to 1 liter

Autoclave for 20 min at 120 °C

Let cool to ca. 55 °C before adding antibiotics

Store at room temperature or 4 °C


Prepare LB as described above, but add before autoclaving:

1,5 % Agar 15 g Agar

Materials needed:

Tryptone (# T1332.1000) by Duchefa
Yeast Extract (# Y1333.1000) by Duchefa

Known Issues:

References and Comments:

This is a known recipe as it can be found for example in "Molecular Cloning: A Laboratory Manual".

How to cite this page in publications:

This document can be cited like this:

Untergasser A. “Media - LB (Luria-Bertani)” Untergasser's Lab. Summer 2006 (include here the date when you accessed these page).

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This article is copyrighted. Please do not reproduce this article in whole or part, in any form, without obtaining my written permission.